mouse integrin αvβ3  (R&D Systems)

Journal: Oncology Letters

Article Title: Bone sialoprotein stimulates cancer cell adhesion through the RGD motif and the αvβ3 and αvβ5 integrin receptors

doi: 10.3892/ol.2024.14675

Figure Lengend Snippet: Effect of anti-αvβ3 or anti-αvβ5 antibody on cancer cell adhesion to BSP, as determined by the alamarBlue ® assay. (A) MDA-MB-231 (n=4), (B) PC-3 (n=4) and (C) NCI-H460 (n=4) cells were incubated for 60 min at 37°C in the presence of 10 µg/ml isotype control IgG1, 10 µg/ml anti-αvβ3 antibody or 10 µg/ml anti-αvβ5 antibody. Afterwards, cells were cultured for 2 h on BSP-coated plates and attached cells were examined. Data are reported as percentages compared with 10 µg/ml isotype control IgG1 (100%). Error bars represent the standard deviation. **P<0.01; ****P<0.0001 vs. negative control IgG1. BSP, bone sialoprotein; ns, not significant.

Article Snippet: Anti-integrin αvβ3 mouse monoclonal antibody, (clone LM609; catalogue no. MAB1976Z) and anti-integrin αvβ5 mouse monoclonal antibody (clone P1F6; catalogue no. MAB1961Z) were obtained from Millipore (Merck KGaA).

Techniques: Alamar Blue Assay, Incubation, Control, Cell Culture, Standard Deviation, Negative Control

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer

doi: 10.1016/j.matbio.2023.11.003

Figure Lengend Snippet: αVβ3 integrin inhibition by the LM609 antibody reduces NEPrCa LuCaP tumor growth. LuCaP 145.2 and LuCaP 173.1 bits were implanted subcutaneously into CB-17 SCID mice. Mice carrying tumors of ~100–150 mm3 were treated intraperitoneally twice weekly for 3 weeks, with LM609 (LuCaP 145.2, n = 5; LuCaP 173.1, n = 5); IgG1 (LuCaP 145.2, n = 5; LuCaP 173.1, n = 5) or PBS (LuCaP 145.2, n = 5; LuCaP 173.1, n = 5). ( A ) LuCaP 145.2 and ( B ) LuCaP 173.1 xenograft volumes were measured twice weekly during treatment. P-values are indicated in the figure. Significance was calculated using the Mann-Whitney test.

Article Snippet: The following Abs were used for the adhesion assays: mouse monoclonal against human αVβ3 integrin LM609 (MAB1976, Millipore) and non-immune mouse monoclonal IgG1 (BE0083, Bio X Cell).

Techniques: Inhibition, MANN-WHITNEY

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer

doi: 10.1016/j.matbio.2023.11.003

Figure Lengend Snippet: Estimated geometric mean ratios by group - LuCaP 145.2.

Article Snippet: The following Abs were used for the adhesion assays: mouse monoclonal against human αVβ3 integrin LM609 (MAB1976, Millipore) and non-immune mouse monoclonal IgG1 (BE0083, Bio X Cell).

Techniques:

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer

doi: 10.1016/j.matbio.2023.11.003

Figure Lengend Snippet: Pairwise comparisons of groups – tumor growth rates – LuCaP 173.1.

Article Snippet: The following Abs were used for the adhesion assays: mouse monoclonal against human αVβ3 integrin LM609 (MAB1976, Millipore) and non-immune mouse monoclonal IgG1 (BE0083, Bio X Cell).

Techniques:

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer

doi: 10.1016/j.matbio.2023.11.003

Figure Lengend Snippet: Pairwise comparisons of groups – tumor growth rates - LuCaP 145.2.

Article Snippet: The following Abs were used for the adhesion assays: mouse monoclonal against human αVβ3 integrin LM609 (MAB1976, Millipore) and non-immune mouse monoclonal IgG1 (BE0083, Bio X Cell).

Techniques:

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer

doi: 10.1016/j.matbio.2023.11.003

Figure Lengend Snippet: NgR2 expression increases cell adhesion to αVβ3 ligands. ( A-D ) DU145 NgR2 cells (+) or DU145 Mock cells not expressing NgR2 (−) were seeded (5 × 10 4 cells/well, 3 replicates) for 1 h on ECM proteins (fibrinogen, vitronectin, collagen, or laminin) or BSA (1 %) coated wells. (A) Fibrinogen (50 μg/mL) adhesion. (B) Vitronectin (5 μg/mL) adhesion. (C) Collagen (50 μg/mL) adhesion. (D) Laminin (10 μg/mL) adhesion. ( E ) DU145 NgR2 cells were seeded (5 × 10 4 cells/well, 2 replicates) for 1 h on ECM proteins (fibrinogen 15 μg/mL or laminin 10 μg/mL) or BSA (1 %) coated wells. Wells were pre-incubated with LM609 (20 μg/mL); treatment with IgG1 (20 μg/mL) or adhesion buffer alone (−) were used as controls. ( A-E ) Bar graphs represent the degree of cell adhesion quantified as optical density (O.D.) of crystal violet staining measured at 600 nm. The values are presented as mean ± standard error of the mean (SEM); P values were calculated using t -test ( n = 3 for each condition).

Article Snippet: The following Abs were used for the adhesion assays: mouse monoclonal against human αVβ3 integrin LM609 (MAB1976, Millipore) and non-immune mouse monoclonal IgG1 (BE0083, Bio X Cell).

Techniques: Expressing, Incubation, Staining

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer

doi: 10.1016/j.matbio.2023.11.003

Figure Lengend Snippet: IB analysis of prostate cancer patient-derived small extracellular vesicles. ( A ) Lysates from density gradient-isolated sEVs from plasma of the following PrCa patients: A, B, C, D, E and F. Fractions six to eight were pooled and preparations from two different patients were combined. IB analysis (reducing conditions) for expression of NgR2 and CD9. PC3 total cell lysate (TCL) was used as a positive control for Calnexin. ( B ) Lysates from density gradient-isolated sEVs in fractions one to ten obtained from plasma of PrCa patient G. IB analysis (reducing conditions) for expression of αVβ3 integrin, NgR2, Syntenin and CD9. ( C ) Lysates from density gradient-isolated sEVs in fractions one to ten obtained from plasma of PrCa patient H. IB analysis (reducing conditions) for expression of αVβ3 integrin, NgR2 and TSG101.

Article Snippet: The following Abs were used for the adhesion assays: mouse monoclonal against human αVβ3 integrin LM609 (MAB1976, Millipore) and non-immune mouse monoclonal IgG1 (BE0083, Bio X Cell).

Techniques: Derivative Assay, Isolation, Expressing, Positive Control

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer

doi: 10.1016/j.matbio.2023.11.003

Figure Lengend Snippet: Estimated geometric mean ratios by group – LuCaP 173.1.

Article Snippet: The following Abs were used for the adhesion assays: mouse monoclonal against human αVβ3 integrin LM609 (MAB1976, Millipore) and non-immune mouse monoclonal IgG1 (BE0083, Bio X Cell).

Techniques: